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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Blood Banking | 15–20% | - Blood group systems and immunology - Blood products and components - Transfusion practice and testing |
| Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question #1
In radioimmunassay, a fixed concentration of labeled antigen is incubated with a constant amount of antiserum such that the concentration of antigen binding sites on the antibody is limiting. If unlabeled antigen is added to this system, there is competition between labeled antigen and unlabeled antigen for the limited and constant number of binding sites on the antibody, and thus the amount of labeled antigen bound to antibody will decrease as the concentration of unlabeled antigen increases.
Which of the following choices is correct when describing the principles of competitive radioimmunoassay procedures?
A. antibody will react with labeled antigen only
B. antibody will react directly with radioactive labeled immunoglobulin
C. antibody will react preferentially with the unlabeled antigen
D. antibody will react equally with labeled and unlabeled antigen
E. antibody will react preferentially with the labeled antigen
Question #2
The normal pH of blood is 7.40. In order for most metabolic processes to take place, the pH must remain within a narrow range close to this value. The range is usually defined in adults as 7.35-7.45. If blood pH falls below 7.35, the blood becomes too acidic (acidosis). If blood rises above 7.45, the blood is too alkaline (alkalosis).
As blood pH decreases, the kidneys will retain bicarbonate (HCO3-) from the glomerular filtrate; therefore, bicarbonate is increased. However, in this case, the increased HCO3- could not compensate for the markedly elevated pCO2 (the respiratory component) and the condition that results is uncompensated respiratory acidosis.
What condition is indicated by the following blood gas results: Bicarbonate = 32 mmol/L (Normal = 22 - 26 mmol/L); pCO2 = 65 mm Hg (Normal = 35 - 45 mmHg); pH = 7.28 (normal = 7.35 - 7.45)
A. Healthy condition
B. Compensated metabolic acidosis
C. Uncompensated metabolic acidosis
D. Uncompensated respiratory acidosis
Question #3
What are the certification requirements for clinical laboratory professionals?
A. on the job training
B. college degree
C. national certification exam
D. college degree and a national certification exam
Question #4
The increase in inflammatory cytokines and the abnormal lipids and lipid levels often present in metabolic syndrome increase the risk of cardiovascular disease. Adiponectin is decreased and increases in angiotensinogen lead to hypertension.
Chemistry
Which of the following statements is true regarding cardiovascular disease and adipose tissue? Please select all correct answers
A. A state of abnormal lipids and lipid levels found in metabolic syndrome contribute to the development of cardiovascular disease
B. Increased synthesis and secretion of PAI-1 in metabolic syndrome contributes to cardiovascular disease by increasing blood pressure
C. Inflammatory cytokines, synthesized and released by adipose tissue, contribute to the development of cardiovascular disease
D. Obesity in metabolic syndrome contributes to the development of cardiovascular disease by increased synthesis and secretion of adiponectin
Question #5
A blood culture to evaluate septicemia is performed in:
A. toxicology.
B. microbiology.
C. urinalysis.
D. hematology.
Solutions:
| Question #1 Correct Answer: D | Question #2 Correct Answer: C | Question #3 Correct Answer: D | Question #4 Correct Answer: A,C | Question #5 Correct Answer: B |







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